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Add 100 μL of bead slurry to the lysate Incubate for 1030 min at 4°C with gentle agitation Spin in microcentrifuge at 14,000 x g at 4°C for 10 min Discard bead pellet and keep supernatant for immunoprecipitation To increase the yield, the beads can be washed 1 or 2 more times in lysis buffer, and the supernatants collected together

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Add 100 μL of bead slurry to the lysate Incubate for 1030 min at 4°C with gentle agitation Spin in microcentrifuge at 14,000 x g at 4°C for 10 min Discard bead pellet and keep supernatant for immunoprecipitation To increase the yield, the beads can be washed 1 or 2 more times in lysis buffer, and the supernatants collected together

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